服務熱線
15201163601
-
詳細分析Chemgenes寡核苷酸定點誘變技術性
發布時間： 2021-07-27 點擊次數： 882次Chemgenes寡核苷酸是一類僅有50個下列堿基的挎包多肽鏈的統稱，它能夠非常容易地和他們的相輔相成鏈連接，因此常見來做為探頭明確DNA或RNA的構造，常常用以基因芯片、電泳原理、瑩光原位雜交等全過程中。Chemgenes寡核苷酸還可以與別的多肽鏈開展混種。Chemgenes寡核苷酸定點誘變技術性：是由澳大利亞的微生物科學家M·阿詩丹頓(Michael Smith，1932—)創造發明的。其基本基本原理以下：運用寡聚多肽鏈開展DNA的定點誘變時，最先要把帶有待突然變化的DNA片斷段復制到MI3噬菌體媒介中，MI3噬菌體的正鏈能夠感染具備微絨毛的病菌，并在病菌身體開展拷貝后，以發芽的方式產生新的含有正鏈DNA的噬菌體。而留存在菌身體的則是雙鏈情況的拷貝型MI3。受MI3噬菌體感染的病菌壽元速率緩減，在病菌細胞培養皿上面產生全透明的噬牙斑菌。將目地DNA插進到拷貝型MI3的多克隆位點上，去轉染病菌，獲取單鏈DNA做為突然變化的模版。依據需要設計方案并生成含有突然變化多肽鏈編碼序列的寡聚多肽鏈引物，使與含有目地DNA的多肽鏈MI3模版混種，隨后添加DNA聚合酶和4種脫氧核糖多肽鏈，使混種上的突然變化引物拓寬，并且用DNA連接酶使新合成的DNA成環形，再去轉染病菌。能用DNA序列剖析的方式從所獲得的噬菌體中挑選出含有突然變化DNA序列的突變體。在制取出帶有突變體的拷貝型DNA后，可以用突然變化的DNA片段換置未突然變化的DNA相對的區間，進而獲得詳細的DNA突變體。
產品中心
Products
-
化學試劑
Origene parkell pan-biotech PAN lexogen Lampire labtidebiotech laborator labome ABI enzolifesciences emsdiasum EtonBioscience Equitech-bio kirgen invivogen integralmolecular Instech IndoorBiotechnologies INCELL IMMUSMOL G-Biosciences IMMUNOLOGICALS idtdna IMMUNODX ILLUMINA Immunodiagnostics IBALifeSciences Hypoxyprobe Horizon Cygnus Technologies Hellobio Greiner Cell Signaling Glycobiotech Corning FST Evrogen eurofins BioFire Defense biodatacorp atlasantibodies ATCC Astartebio ArcticZymes aobious anshlabs Advanced ChemTech Advaced Cell Diagnostics AdvanDx Agar Scientific ACBR TRc polyscience Fluka Gene Bridges fortebio Biocrick Fisher bioflux biomax beckman AmericanElements MOLTOX Permagen USA Scientific Chemgenes寡核苷酸 Asuragen vmrd抗體 Thermo asusragen AmplideX toxin polyplus signagen Berry&Associates ALZET滲透泵 alzet abcr abvector SGI-DNA Goldbio Iduron Sgidna Chromsystems Coriell GE Chemgenes Matreya Streck VMRD Sigma Cerilliant Innovative Cell Technologies Diagenode Trilinkbiotech Medkoo life diagnostics Gibco Neuromab全國總代 Novus BioLegend Bethyl Toxin Technology Listlabs Seracare 寶利嘉 賽默飛 安迪生物 行業專用試劑
-
生物試劑
Biosharp Sciex Seahorse Proteintech DiaMag complementtech PeproTech hooke Nzytech Pierce pepnet pelfreez PALL ozbiosciences OXOID ORFLO ommscientific omega nanopore ncbi NexcelomBioscience Mpbio MultiSciences MyBioSource Musechem MiltenyiBiotec minerva-biolabs microspheres-nanospheres microfil microbix modiquestresearch MOBITEC mercodia merckmillipore medicago JPT kerafast Kangchen Malvern magen Kapa Macrocyclics Macherey mabtech Lucigen leica Lifespan lifesensors lifeline life-ilab Lifecore life Licor advancedchemtech Affinity Abgent Abmgood ActiveMotif Acros Acrobiosystems enzymatics epicypher Elastin elabscience Echelon eBioscience eastcoast eaglebio E&K SCIENTIFIC ekfusa duran DSHB Dharmacon DGRC Dako Cypex comptech Encorbio Jetbiofil JCRBcellbank ISOSCIENCES INVIVOCHEM iris-biotech Full Moon BioSystems GBI FormnMax Finescience Favorgen CordenPharma CIL Chromadex Clodronateliposomes Chromogenix Chiron chemservice cellvis cellutron CELLTREAT cellscript cellbiolabs cellecta cedarlane cdnisotopes carbosynth cambio calbioreagents Buchem Boston Cellron Bioworld Biowest Biotium biostatus BiosPacific Biosearch BIOplastics Biooscientific BioPAL Biomol BIOMED Biomatik bioline Biocoat Bioclone Biocision biochain華新康信 biocell Biocare BIAseparations BDpharmingen Baseclick Badrilla B2S Life Sciences Axon Medchem AXIS-SHIELD avivasysbio avidity AVES-LABS Anatrace Americanbio AlphaDiagnoesticInternational Applied Genetics Laboratories Alstem Alomone AcceGen Akina AdvancedBioMatriX Adipogen Accuratechemical Academybiomed Abcam Zymo Research Addexbio ABL Abbexa Aablocks ZeptoMetrix Tocris TAKARA Spherotech Santa Roche Nist PBL MRC Holland rd-biotech Cellgs LC-LAB Greatcell Energy Hyclone Greerlabs GlycoSyn GlycoTech EY laboratories Cayman CanSyn Bioxcell Bio－Rad Agdia Abclonal amsbio auditmicro avantilipids Alsachim HTDialysis jetPRIME Carna Biosciences FItzgerald